- Author
-
M.M. van der Stoel
- Title
- Forces that control endothelial adhesion in angiogenesis
- Supervisors
- Co-supervisors
- Award date
- 9 June 2021
- Number of pages
- 215
- ISBN
- 9789464232547
- Document type
- PhD thesis
- Faculty
- Faculty of Medicine (AMC-UvA)
- Abstract
-
Angiogenesis, the formation of new blood vessels from preexisting ones, is regulated by collective migration of endothelial cells, by dynamic remodeling of their cell – cell and cell – extracellular matrix adhesions. During angiogenesis, the mechanical forces subjected to the endothelial layer change, including matrix rigidity, hemodynamic forces and actin cytoskeletal contractility. The endothelial adhesions can sense and transmit these forces into biochemical signaling, resulting in changes in gene expression, adhesion, migration, proliferation and angiogenesis. In this thesis, we examined how different endothelial signaling events regulate sprouting angiogenesis via modulation of cell – cell and cell- extracellular matrix adhesions. Using in vitro and in vivo angiogenic sprouting models we describe how endothelial functions are modulated by internal processes such as cholesterol biosynthesis and cytoskeletal-mechanotransduction, but also by external stimuli, like extracellular matrix stiffening and hemodynamic forces, and the implications that these have for angiogenic processes. Overall, this thesis contributes to a better understanding of the endothelial adhesion mechanotransductory processes during angiogenesis.
- Persistent Identifier
- https://hdl.handle.net/11245.1/f1d0c264-cebb-41dd-9268-eb61d84cb334
- Downloads
-
Thesis (complete)
Front matter
Chapter 1: General introduction
Chapter 2: Endothelial YAP/TAZ signaling in angiogenesis and tumor vasculature
Chapter 3: DLC1 is a direct target of activated YAP/TAZ that drives collective migration and sprouting angiogenesis
Chapter 4: The Rho GAP DLC1 acts as a negative feedback sensor for YAP in angiogenic sprouting
Chapter 5: Vinculin controls endothelial cell junction dynamics during vascular lumen formation
Chapter 6: Vinculin strengthens the endothelial barrier during vascular development
Chapter 7: The MARCH6-SQLE axis controls endothelial cholesterol homeostasis and angiogenic sprouting
Chapter 8: General discussion
Summary; Samenvatting; Author contributions; List of publications; PhD portfolio; Curriculum vitae; Acknowledgements
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