- Author
-
R.J. de Vries
- Title
- Extended preservation and viability assessment of the liver for transplantation
- Supervisors
-
T.M. van Gulik
- Co-supervisors
-
M.K. Uygun
- Award date
- 23 April 2021
- Number of pages
- 335
- ISBN
- 9789464164473
- Document type
- PhD thesis
- Faculty
- Faculty of Medicine (AMC-UvA)
- Abstract
-
Liver transplantation is the only cure for patients with end-stage liver failure, however its success is seriously curtailed by the shortage of suitable donor livers. Restrictions in organ preservation substantially limit the pool of usable donor livers. The long-term goal of this thesis is to reduce deaths due to the organ shortage and improve outcomes after liver transplantation by developing improved organ preservation methods that can extend the preservation duration and advance viability assessment of liver grafts. This thesis presents novel strategies for subzero organ preservation and innovative methods to assess liver viability during machine perfusion. These were developed and tested in various pre-clinical animal and human liver studies. Results of these studies showed that subzero preservation strategies can extend the preservation duration and new methods to assess liver viability hold great promise to increase the number of donor livers that can be transplanted. The findings of this thesis justify translation of these innovations to the clinic to help reduce deaths due to the shortage of donor livers for transplantation.
- Persistent Identifier
- https://hdl.handle.net/11245.1/bf6dc1f1-4813-480b-8610-4ed1e8a9477a
- Downloads
-
Thesis (complete)
Front matter
Chapter 1: General introduction and outline of this thesis
Chapter 2: Systems engineering the organ preservation process for transplantation
Chapter 3: Supercooling of human livers to extend the preservation time
Chapter 4: Sub-zero non frozen preservation of human livers in the supercooled state
Chapter 5: Partial freezing of rat livers: A nature-inspired approach for organ banking
Chapter 6: Bulk droplet vitrification: An approach to improve largescale hepatocyte cryopreservation outcome
Chapter 7: Bulk droplet vitrification for primary hepatocyte preservation
Chapter 8: Pumping new life in old ideas: Preservation and rehabilitation of the liver using ex situ machine perfusion
Chapter 9: Viability testing of discarded livers with normothermic machine perfusion: Alleviating the organ shortage outweighs the cost
Chapter 10: Cell release during perfusion reflects cold ischemic injury in rat livers
Chapter 11: Non-invasive quantification of the mitochondrial redox state in livers
Chapter 12: Summary, general discussion and future perspectives
Hoofdstuk 12: Nederlandse samenvatting, discussie, en toekomstperspectieven
Abbreviations; PhD portfolio; List of publication and patents; List of contributing authors; Acknowledgements; Curriculum vitae
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