- Author
-
J. Hrdinová
- Title
- ADAMTS13 and thrombotic thrombocytopenic purpura
- Subtitle
- On the edge between hemostasis and autoimmunity
- Supervisors
-
J.J. Voorberg
K. Vanhoorelbeke - Co-supervisors
-
C.P.M. Reutelingsperger
G.A.F. Nicolaes - Award date
- 29 January 2021
- Number of pages
- 231
- ISBN
- 9789464231144
- Document type
- PhD thesis
- Faculty
- Faculty of Medicine (AMC-UvA)
- Abstract
-
Immune thrombotic thrombocytopenic purpura (iTTP) is a hematological condition which presents with thrombocytopenia, hemolytic anemia, neurological symptoms and schistocytes on a blood smear. Although being rare, iTTP is a deadly disease with rapid progression when left untreated. In the past few years, the diagnosis and treatment of iTTP patients has been significantly improved. Nevertheless, the majority of patients suffer from relapses, tiredness, neurological disorders after the first iTTP bout, which cannot be overcome by current treatment strategies.
In the studies included in this thesis we aimed to improve our understanding of the pathophysiology of iTTP in order to be able to design improved, targeted therapies with fewer side effects. In this thesis we discuss the latest advances in our current understanding of the pathophysiology of iTTP. Furthermore, we discuss structural changes in ADAMTS13 and their role in regulation of proteolytic activity towards its substrate von Willebrand factor. Additionally, we discuss possible involvement of ADAMTS13 structural changes in the onset of autoimmunity and place these recent findings into context of other autoimmune diseases. In our research we also identified ADAMTS13 peptides that are preferentially presented on HLA-DR and HLA-DQ of healthy individuals. Furthermore, the immunogenic potential of the identified HLA-DR- and HLA-DQ-presented ADAMTS13 peptides was assessed using in silico methods. In this thesis we also introduced novel treatment strategies for iTTP; based on additional N-glycan insertion on the major autoantibody epitope in the ADAMTS13 spacer domain and designed novel peptide inhibitors of the VWF A1- platelet GPIbα axis. - Persistent Identifier
- https://hdl.handle.net/11245.1/76f46fb0-590d-456f-a069-02064baea3b9
- Downloads
-
Thesis (complete)
Front matter
Chapter 1: General introduction
Chapter 2: Dissecting the pathophysiology of immune thrombotic thrombocytopenic purpura: Interplay between genes and environmental triggers
Chapter 3: Conformational plasticity of ADAMTS13 in immune TTP: A blood-based remake of Dr. Jekyll and Mr. Hyde
Chapter 4: Rational modulator design by exploitation of protein–protein complex structures
Chapter 5: Mass spectrometry-assisted identification of ADAMTS13- derived peptides presented on HLA-DR and HLA-DQ
Chapter 6: N-glycan mediated shielding of ADAMTS13 prevents binding of pathogenic autoantibodies in immune-mediated TTP
Chapter 7: Structure-based design of cyclic glycoprotein Ibα-derived peptides affecting platelet interaction with von Willebrand factor under shear conditions
Chapter 8: General discussion
Appendix; Summary; Samenvatting; PhD portfolio; Publication list; Authors and affiliations; About the author; Acknowledgements/Dankwoord
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